Skip to content

NanoMal’s blood analyzer

QuantumDX. Malaria DNA assay, Covid Sars-Cov2 test

Menu
  • Home
  • ELISA kit
  • Real-time
  • JopLink
  • reverse
  • cDNA
  • Tubes
  • The TECHNOLOGY
  • Contact Us
  • Distributors
Menu
nanomal

Analysis of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay

Posted on May 29, 2021May 4, 2021 by Garrett

Performance of the QPLEX™ Alz plus assay, a novel multiplex kit for screening cerebral amyloid deposition

Background: Alzheimer’s disease (AD) is an irreversible neurodegenerative disease characterized by the hallmark finding of cerebral amyloid deposition. Many researchers have tried to predict the existence of cerebral amyloid deposition by using easily accessible blood plasma samples, but the effectiveness of such strategies remains controversial.

Methods: We developed a new multiplex kit, the QPLEX™ Alz plus assay kit, which uses proteomics-based blood biomarkers to prescreen for cerebral amyloid deposition. A total of 300 participants who underwent Pittsburgh compound B (PiB)-positron emission tomography (PET) which allows imaging of cerebral amyloid deposition were included in this study. We compared the levels of QPLEX™ biomarkers between patients who were classified as PiB-negative or PiB-positive, regardless of their cognitive function. Logistic regression analysis followed by receiver operating characteristic (ROC) curve analysis was performed. The kit accuracy was tested using a randomized sample selection method.

Results: The results obtained using our assay kit reached 89.1% area under curve (AUC) with 80.0% sensitivity and 83.0% specificity. Further validation of the QPLEX™ Alz plus assay kit using a randomized sample selection method showed an average accuracy of 81.5%.

Conclusions: Our QPLEX™ Alz plus assay kit provides preliminary evidence that it can be used as blood marker to predict cerebral amyloid deposition but independent validation is needed.

nanomal
nanomal

FAM-Phe-DAP Serine Protease Assay Kit

abx299013-25tests Abbexa 25 tests
EUR 582

SR-101-Leu-CMK FLISP™ Assay Kit

KF17321 Neuromics 100 Tests
EUR 695

FAM-Leu-CMK Serine Protease Assay Kit

abx299011-25tests Abbexa 25 tests
EUR 566

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN CORNING 1/pk
EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Polycaspase Assay Kit, green

KF17200 Neuromics 25 Tests
EUR 304

SR-101-Phe-CMK FLISP™ Assay Kit

KF17318 Neuromics 25 Tests
EUR 291

SR-101-Phe-CMK FLISP™ Assay Kit

KF17319 Neuromics 100 Tests
EUR 695

Caspase 1 Assay Kit, green

KF17201 Neuromics 25 Tests
EUR 325

Caspase 2 Assay Kit, green

KF17202 Neuromics 25 Tests
EUR 304

Caspase 6 Assay Kit, green

KF17204 Neuromics 25 Tests
EUR 304

Caspase 8 Assay Kit, green

KF17205 Neuromics 25 Tests
EUR 304

Caspase 9 Assay Kit, green

KF17206 Neuromics 25 Tests
EUR 304

Caspase 10 Assay Kit, green

KF17207 Neuromics 25 Tests
EUR 304

Caspase 13 Assay Kit, green

KF17208 Neuromics 25 Tests
EUR 304

Malachite Green Phosphate Assay Kit

abx298878-100Assays Abbexa 100 Assays
EUR 378

Malachite Green Phosphate Assay Kit

POMG-25H BioAssay Systems 2500
EUR 275
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Malachite Green Phosphate Assay Kit

Z5030012 Biochain 2,500 assays
EUR 342
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Caspase 3 & 7 Assay Kit, green

KF17203 Neuromics 25 Tests
EUR 304

EZCell? Phagocytosis Assay Kit (Green Zymosan)

K397-100 Biovision
EUR 430

FAM-Phe-CMK Serine Protease Assay Kit

abx299010-25tests Abbexa 25 tests
EUR 566

FAM-cAMP PDE IV substrate *Green Fluorescence*

13602 AAT Bioquest 0.5 umol
EUR 306

FAM-cGMP PDE V substrate *Green Fluorescence*

13604 AAT Bioquest 0.5 umol
EUR 306

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342 Cell Biolabs 96 assays
EUR 635
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342-5 Cell Biolabs 5 x 96 assays
EUR 2549
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)

STA-349 Cell Biolabs 192 assays
EUR 537
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

EZCell? Phagocytosis Assay Kit (Green E. coli)

K963-100 Biovision
EUR 430

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-1895.0001 Bachem 1.0mg
EUR 321
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-1895.0005 Bachem 5.0mg
EUR 1192
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

11401 AAT Bioquest 200 Tests
EUR 219

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2350.0001 Bachem 1.0mg
EUR 368
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2350.0005 Bachem 5.0mg
EUR 1385
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2

M-2670.0001 Bachem 1.0mg
EUR 334
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2

M-2670.0005 Bachem 5.0mg
EUR 1240
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

H-2638.0001 Bachem 1.0mg
EUR 236
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

H-2638.0005 Bachem 5.0mg
EUR 853
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Dap/ Rat Dap ELISA Kit

ELI-31052r Lifescience Market 96 Tests
EUR 886

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 AAT Bioquest 500 Tests
EUR 219

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

12601 AAT Bioquest 500 Tests
EUR 306

Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence*

13512 AAT Bioquest 100 Tests
EUR 306

Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

13601 AAT Bioquest 200 Tests
EUR 219

Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*

22786 AAT Bioquest 500 Tests
EUR 219

Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence*

22849 AAT Bioquest 50 Tests
EUR 306

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T Cell Biolabs 20 assays
EUR 345
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347 Cell Biolabs 96 assays
EUR 612
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347-5 Cell Biolabs 5 x 96 assays
EUR 2428
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K452-100 Biovision
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K453-100 Biovision
EUR 490

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K497-100 Biovision
EUR 490

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2225.0001 Bachem 1.0mg
EUR 371
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2225.0005 Bachem 5.0mg
EUR 1321
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2145.0001 Bachem 1.0mg
EUR 441
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2145.0005 Bachem 5.0mg
EUR 1675
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

13456 AAT Bioquest 100 tests
EUR 306

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

13500 AAT Bioquest 500 Tests
EUR 219

Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*

13503 AAT Bioquest 500 Tests
EUR 306

Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

13510 AAT Bioquest 100 Tests
EUR 306

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*

16315 AAT Bioquest 100 Tests
EUR 350

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*

22730 AAT Bioquest 200 Tests
EUR 219

Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5524 AAT Bioquest 200 Tests
EUR 219

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

K459-100 Biovision
EUR 550

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2

M-2520.0001 Bachem 1.0mg
EUR 321
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2

M-2520.0005 Bachem 5.0mg
EUR 1192
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*

11503 AAT Bioquest 200 Tests
EUR 263

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*

16060 AAT Bioquest 200 Tests
EUR 306

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*

22798 AAT Bioquest 200 Tests
EUR 219

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

22799 AAT Bioquest 200 Tests
EUR 306

Amplite™ Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5528 AAT Bioquest 200 Tests
EUR 219

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size

STA-347-T Cell Biolabs 20 assays
EUR 334
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K560-100 Biovision
EUR 490

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K592-100 Biovision
EUR 490

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K714-100 Biovision
EUR 512

6-FAM

HY-66021 MedChemExpress 500mg
EUR 312

5-FAM

HY-66022 MedChemExpress 10mM/1mL
EUR 126

5-FAM

20-abx095073 Abbexa
  • EUR 523.00
  • EUR 370.00
  • EUR 1274.00
  • 1 g
  • 500 mg
  • 5 g

DAP Antibody

1-CSB-PA262326 Cusabio
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

DAP Antibody

1-CSB-PA01034A0Rb Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:50-1:200

DAP Antibody

1-CSB-PA006496GA01HU Cusabio
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA

DAP Antibody

1-CSB-PA007640 Cusabio
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

DAP Antibody

1-CSB-PA170450 Cusabio
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

DAP Antibody

35710-100ul SAB 100ul
EUR 252

DAP siRNA

20-abx913570 Abbexa
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP siRNA

20-abx913571 Abbexa
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP siRNA

20-abx901410 Abbexa
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP antibody

38736-100ul SAB 100ul
EUR 252

DAP Antibody

43505-100ul SAB 100ul
EUR 252

DAP Antibody

ABD7628 Lifescience Market 100 ug
EUR 438

DAP Antibody

DF7628 Affbiotech 200ul
EUR 304
Description: DAP Antibody detects endogenous levels of total DAP.

DAP antibody

70R-16735 Fitzgerald 50 ul
EUR 435
Description: Rabbit polyclonal DAP antibody

DAP Peptide

7389P ProSci 0.05 mg
EUR 164.75
Description: (CT) DAP Peptide

DAP antibody

70R-32429 Fitzgerald 100 ug
EUR 327
Description: Rabbit polyclonal DAP antibody

DAP Antibody

7389-002mg ProSci 0.02 mg
EUR 171.82
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

DAP Antibody

7389-01mg ProSci 0.1 mg
EUR 436.42
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
×

Analysis of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay: A Comparative Study of Four Isolation Kits

There is a lack of reliable biomarkers for disorders of the central nervous system (CNS), and diagnostics still heavily rely on symptoms that are both subjective and difficult to quantify. The cerebrospinal fluid (CSF) is a promising source of biomarkers due to its close connection to the CNS. Extracellular vesicles are actively secreted by cells, and proteomic analysis of CSF extracellular vesicles (EVs) and their molecular composition likely reflects changes in the CNS to a higher extent compared with total CSF, especially in the case of neuroinflammation, which could increase blood-brain barrier permeability and cause an influx of plasma proteins into the CSF. We used proximity extension assay for proteomic analysis due to its high sensitivity.

We believe that this methodology could be useful for de novo biomarker discovery for several CNS diseases. We compared four commercially available kits for EV isolation: MagCapture and ExoIntact (based on magnetic beads), EVSecond L70 (size-exclusion chromatography), and exoEasy (membrane affinity). The isolated EVs were characterized by nanoparticle tracking analysis, ELISA (CD63, CD81 and albumin), and proximity extension assay (PEA) using two different panels, each consisting of 92 markers. The exoEasy samples did not pass the built-in quality controls and were excluded from downstream analysis.

The number of detectable proteins in the ExoIntact samples was considerably higher (~150% for the cardiovascular III panel and ~320% for the cell regulation panel) compared with other groups. ExoIntact also showed the highest intersample correlation with an average Pearson’s correlation coefficient of 0.991 compared with 0.985 and 0.927 for MagCapture and EVSecond, respectively. The median coefficient of variation was 5%, 8%, and 22% for ExoIntact, MagCapture, and EVSecond, respectively. Comparing total CSF and ExoIntact samples revealed 70 differentially expressed proteins in the cardiovascular III panel and 17 in the cell regulation panel. To our knowledge, this is the first time that CSF EVs were analyzed by PEA. In conclusion, analysis of CSF EVs by PEA is feasible, and different isolation kits give distinct results, with ExoIntact showing the highest number of identified proteins with the lowest variability.

 

A simple and rapid algal assay kit to assess toxicity of heavy metal-contaminated water

This study presents a novel algal-based toxicity test suitable for simple and rapid assessment of heavy metal (Hg2+, Cr6+, Cd2+, Pb2+, or As3+)-contaminated water. A closed-system kit-type algal assay was developed using Chlorella vulgaris. Toxicity was assessed by oxygen evolution in the gaseous phase of the assay kits, which was measured via a needle-type oxygen sensor. Initial cell density, light intensity, and exposure time that enabled favorable test performance for the algal assay kits were 103 cells/mL, 250 μmol m-2s-1, and 18 h, respectively.

Results from the heavy metal toxicity tests demonstrate that Hg2+, Cr6+, Cd2+, and Pb2+ are more toxic in inhibiting algal photosynthetic activity than As3+. The 18 h half-maximum effective concentrations (EC50) for Hg2+, Cr6+, Cd2+, Pb2+, and As3+ were determined to be 31.3 ± 0.5, 179.6 ± 7.5, 301.3 ± 6.1, 476.1 ± 10.5, and 2184.1 ± 31.1 μg/L, respectively. A strong correlation between oxygen concentrations in the headspace of the assay kits and chlorophyll a production indicates that oxygen evolution in the gaseous phase is able to represent algal photosynthetic activity and serve as the end-point in algal toxicity tests. High test sensitivity and reproducibility as well as an easy test protocol and rapid processing time make the algal assay kit a suitable tool for simple and rapid toxicity testing of heavy metal-contaminated water.

Sperm chromatin structure assay versus sperm chromatin dispersion kits: Technical repeatability and choice of assisted reproductive technology procedure

 

Objective: The sperm DNA fragmentation index (DFI) guides the clinician’s choice of an appropriate assisted reproductive technology (ART) procedure. The DFI can be determined using commercially available methodologies, including sperm chromatin dispersion (SCD) kits and sperm chromatin structure assay (SCSA). Currently, when DFI is evaluated using SCD kits, the result is analyzed in reference to the SCSA-derived threshold for the choice of an ART procedure. In this study, we compared DFI values obtained using SCSA with those obtained using SCD and determined whether the difference affects the choice of ART procedure.

Methods: We compared SCSA to two SCD kits, CANfrag (n=36) and Halosperm (n=31), to assess the DFI values obtained, the correlations between tests, the technical repeatability, and the impact of DFI on the choice of ART.

Results: We obtained higher median DFI values using SCD kits than when using SCSA, and this difference was significant for the CANfrag kit (p<0.001). The SCD kits had significantly higher coefficients of variation than SCSA (p<0.0001). In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) would be chosen for a significantly higher proportion of patients if a decision were made based on DFI derived from SCD rather than DFI determined using SCSA (p=0.003).

Conclusion: Our results indicate that SCD kit-specific thresholds should be established in order to avoid the unnecessary use of IVF/ICSI based on sperm DNA damage for the management of infertility. Appropriate measures should be taken to mitigate the increased variability inherent to the methods used in these tests.

Caspase-1 Activity Assay Kit

AR4003-25Assays BosterBio 25 Assays
EUR 236

Caspase-1 Activity Assay Kit

AR4003-50Assays BosterBio 50 Assays
EUR 340

Caspase 1 Assay Kit, green

KF17201 Neuromics 25 Tests
EUR 325

Caspase-1 Fluorometric Assay Kit

K2010-200 ApexBio 200 assays
EUR 696

Caspase-1 Fluorometric Assay Kit

K2010-25 ApexBio 25 assays
EUR 238

Caspase-1 Fluorometric Assay Kit

K2010-400 ApexBio 400 assays
EUR 1086

Caspase-1 Colorimetric Assay Kit

K2011-200 ApexBio 200 assays
EUR 696

Caspase-1 Colorimetric Assay Kit

K2011-25 ApexBio 25 assays
EUR 238

Caspase-1 Colorimetric Assay Kit

K2011-400 ApexBio 400 assays
EUR 1086

Caspase-1 Fluorometric Assay Kit

K110-100 Biovision
EUR 479

Caspase-1 Fluorometric Assay Kit

K110-200 Biovision
EUR 620

Caspase-1 Fluorometric Assay Kit

K110-25 Biovision
EUR 207

Caspase-1 Fluorometric Assay Kit

K110-400 Biovision
EUR 958

Caspase-1 Colorimetric Assay Kit

K111-100 Biovision
EUR 479

Caspase-1 Colorimetric Assay Kit

K111-200 Biovision
EUR 620

Caspase-1 Colorimetric Assay Kit

K111-25 Biovision
EUR 207

Caspase-1 Colorimetric Assay Kit

K111-400 Biovision
EUR 958

Caspase 1 Fluorometric Assay Kit

55R-1272 Fitzgerald 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 Fitzgerald 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-1 Biotium 1KIT
EUR 100
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

30012-1 Biotium 1KIT
EUR 100
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 3 Assay Kit

20-abx294003 Abbexa
  • EUR 1255.00
  • EUR 457.00
  • EUR 770.00
  • 100 tests
  • 20 tests
  • 50 tests

Poly-Caspase Assay Kit

20-abx299016 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 6 Assay Kit

20-abx299018 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Poly-Caspase Assay Kit

abx299022-2550tests Abbexa 25-50 tests
EUR 692

Poly-Caspase Assay Kit

20-abx299025 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 2 Assay Kit

abx299026-25tests Abbexa 25 tests
EUR 582

Caspase 10 Assay Kit

abx299027-100tests Abbexa 100 tests
EUR 1302

Caspase 9 Assay Kit

20-abx299035 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 12 Assay Kit

55R-1290 Fitzgerald 25 assays
EUR 328
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Glycerol Cell-Based Assay Kit

K977-100 Biovision
EUR 490

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 Biotium 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-2 Activity Assay Kit

AR4004-100Assays BosterBio 100 Assays
EUR 514

Caspase-2 Activity Assay Kit

AR4004-25Assays BosterBio 25 Assays
EUR 236

Caspase-2 Activity Assay Kit

AR4004-50Assays BosterBio 50 Assays
EUR 340

Caspase 3/7 Assay Kit

20-abx299017 Abbexa
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299024-2550tests Abbexa 25-50 tests
EUR 692

Caspase 3/7 Assay Kit

20-abx299028 Abbexa
  • EUR 1193.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 2 Assay Kit, green

KF17202 Neuromics 25 Tests
EUR 304

Caspase 6 Assay Kit, green

KF17204 Neuromics 25 Tests
EUR 304

Caspase 8 Assay Kit, green

KF17205 Neuromics 25 Tests
EUR 304

Caspase 9 Assay Kit, green

KF17206 Neuromics 25 Tests
EUR 304

Caspase 10 Assay Kit, green

KF17207 Neuromics 25 Tests
EUR 304

Caspase 13 Assay Kit, green

KF17208 Neuromics 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 Neuromics 25 Tests
EUR 304

Caspase-3 Fluorometric Assay Kit

K2007-200 ApexBio 200 assays
EUR 696

Caspase-3 Fluorometric Assay Kit

K2007-25 ApexBio 25 assays
EUR 238

Caspase-3 Fluorometric Assay Kit

K2007-400 ApexBio 400 assays
EUR 1086

Caspase-3 Colorimetric Assay Kit

K2008-200 ApexBio 200 assays
EUR 696

Caspase-3 Colorimetric Assay Kit

K2008-25 ApexBio 25 assays
EUR 238

Caspase-3 Colorimetric Assay Kit

K2008-400 ApexBio 400 assays
EUR 1086

Caspase-8 Fluorometric Assay Kit

K2012-200 ApexBio 200 assays
EUR 696

Caspase-8 Fluorometric Assay Kit

K2012-25 ApexBio 25 assays
EUR 238

Caspase-8 Fluorometric Assay Kit

K2012-400 ApexBio 400 assays
EUR 1086

Caspase-8 Colorimetric Assay Kit

K2013-200 ApexBio 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-25 ApexBio 25 assays
EUR 238

Caspase-8 Colorimetric Assay Kit

K2013-400 ApexBio 400 assays
EUR 1086

Caspase-6 Fluorometric Assay Kit

K2014-200 ApexBio 200 assays
EUR 696

Caspase-6 Fluorometric Assay Kit

K2014-25 ApexBio 25 assays
EUR 238

Caspase-6 Fluorometric Assay Kit

K2014-400 ApexBio 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-200 ApexBio 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-25 ApexBio 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-400 ApexBio 400 assays
EUR 1101

Caspase-2 Fluorometric Assay Kit

K2016-200 ApexBio 200 assays
EUR 696

Caspase-2 Fluorometric Assay Kit

K2016-25 ApexBio 25 assays
EUR 238

Caspase-2 Fluorometric Assay Kit

K2016-400 ApexBio 400 assays
EUR 1114

Caspase-2 Colorimetric Assay Kit

K2017-200 ApexBio 200 assays
EUR 725

Caspase-2 Colorimetric Assay Kit

K2017-25 ApexBio 25 assays
EUR 251

Caspase-2 Colorimetric Assay Kit

K2017-400 ApexBio 400 assays
EUR 1156

Caspase-9 Fluorometric Assay Kit

K2018-200 ApexBio 200 assays
EUR 669

Caspase-9 Fluorometric Assay Kit

K2018-25 ApexBio 25 assays
EUR 238

Caspase-9 Fluorometric Assay Kit

K2018-400 ApexBio 400 assays
EUR 1114

Caspase-9 Colorimetric Assay Kit

K2019-200 ApexBio 200 assays
EUR 696

Caspase-9 Colorimetric Assay Kit

K2019-25 ApexBio 25 assays
EUR 251

Caspase-9 Colorimetric Assay Kit

K2019-400 ApexBio 400 assays
EUR 1156

Caspase-12 Fluorometric Assay Kit

K2150-100 ApexBio 100 assays
EUR 529

Caspase-12 Fluorometric Assay Kit

K2150-25 ApexBio 25 assays
EUR 251

Caspase-5 Fluorometric Assay Kit

K2195-100 ApexBio 100 assays
EUR 502

Caspase-5 Fluorometric Assay Kit

K2195-200 ApexBio 200 assays
EUR 696

Caspase-5 Fluorometric Assay Kit

K2195-25 ApexBio 25 assays
EUR 238

Caspase-5 Fluorometric Assay Kit

K2195-400 ApexBio 400 assays
EUR 1114

Caspase-5 Colorimetric Assay Kit

K2196-100 ApexBio 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 ApexBio 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-25 ApexBio 25 assays
EUR 251

Caspase-5 Colorimetric Assay Kit

K2196-400 ApexBio 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 ApexBio 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 ApexBio 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-25 ApexBio 25 assays
EUR 251
×

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recent Posts

  • Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular
  • Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay
  • Analysis of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay
  • An Alternative Tool to the Commercialized Kits Used for Severe Acute Respiratory
  • Determining the Need for Repeat Testing of Blood Ethanol Concentration

Recent Comments

    Categories

    • blood
    • Blot
    • cDNA
    • Cultrex
    • ELISA kit
    • Enzyme
    • Real-time
    • Recombinant
    • Uncategorized

    Categories

    • blood
    • Blot
    • cDNA
    • Cultrex
    • ELISA kit
    • Enzyme
    • Real-time
    • Recombinant
    • Uncategorized

    Categories

    • blood
    • Blot
    • cDNA
    • Cultrex
    • ELISA kit
    • Enzyme
    • Real-time
    • Recombinant
    • Uncategorized

    Recent Posts

    • Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular
    • Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay
    • Analysis of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay
    • An Alternative Tool to the Commercialized Kits Used for Severe Acute Respiratory
    • Determining the Need for Repeat Testing of Blood Ethanol Concentration

    Quick Links

    • #25 (no title)
    • Contact Us
    • Distributors
    • Genomic sequencing biosensor
    • Nanomaterials and nanoparticles: Sources and toxicity
    • Prevention malaria deaths through rapid diagnosis
    • The NANOMAL PARTNERS
    • The NANOMAL PROJECT NEWS
    • The TECHNOLOGY
    © 2022 NanoMal’s blood analyzer | Powered by Superbs Personal Blog theme