OptiPrep Density Gradient Medium
Ideally, any density gradient for the isolation of mammalian organelles should only expose the settling biological particles to an increasing concentration of the gradient solute. Therefore, they will experience only increasing density and viscosity, other parameters such as osmolality, pH, ionic strength, and the concentration of important additives (such as EDTA and DTT) must remain as constant as possible. This protocol article describes strategies for the dilution of OptiPrep™ in order to prepare such solutions for mammalian organelles and membranes.
Keywords: gradient solutions, OptiPrep™, iodixanol, density, osmolality, pH, mammalian nuclei, mammalian organelles
This product is manufactured by BioVision, an Abcam company and was formerly called M1248 OptiPrep™ Density Gradient Medium. M1248-250 is the same size as the 250 mL size of ab286850. A sterile and tested ready-to-use solution of 60% iodixanol endotoxin in water with a density of 1.32 g/ml.
Shipped at room temperature. Store at room temperature. Do not freeze. This product is sensitive to air and light and impurities may occur as a result of oxidation from the air or due to metabolism by microbes.
Constituent: 60% Iodixanol