Intended Use: For Research Only
Lymphoprep™ is a ready-to-use, sterile, endotoxin-tested solution for the isolation of suspensions of pure lymphocytes. The solution contains sodium diatrizoate and polysaccharides.
Density: 1.077 ± 0.001 g/ml
Composition: 9.1% (w/v) sodium diatrizoate, 5.7% (w/v) polysaccharide
Osmolality: 290 ± 15 mOsm
Endotoxin: < 1.0 EU/ml
Stability and storage
Lymphoprep™ is stable for 3 years as long as the solution is kept sterile and protected from light. Prolonged exposure to direct sunlight causes the release of iodine from the sodium diatrizoate molecule. This effect is negligible when working with this solution on a day-to-day basis. Lymphoprep™ should be stored between +4°C and +30°C.
Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities less than 1.077 g/ml. Therefore, these cells can be isolated by centrifugation in an isoosmotic medium with a density of 1.077 µg/mL, allowing erythrocytes and PMNs to sediment in the medium while retaining mononuclear cells at the sample/medium interface.
- The described method is fast, simple and reliable and gives excellent results with blood samples from normal individuals and patients.
- For maximum performance, it is important that the blood sample is diluted 1:1 with normal saline before applying it to the gradient.
- Contamination of erythrocytes in the mononuclear cell suspension is usually between 3-10% of the total number of cells.